eSeminars
&
eConference & Webinars
central topics = qPCR
& dPCR &
RNAi &
Molecular-Biology
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This
streaming portal is dedicated to
scientists from the community of
qPCR,
digital PCR, Next Generation
Sequencing (NGS), MicroGenomics (MG)
and
Molecular Diagnostics (MDx). You’ll
find here all the records from
around 350 presentations held
at qPCR & NGS and MG Events in
the
past years – qPCR 2010 in Vienna to
qPCR dPCR & NGS 2019 in
Freising-Weihenstephan, and
MicroGenomics
2014
in Paris.
We provide the presentations via
movie streaming technology in high
quality – high resolution and
perfect sound quality in high speed
– on
any internet browser or mobile
device, including Android or iOS.
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iBiology
iBiology’s mission is to
convey, in the form
of open-access free videos, the
excitement of modern biology and the
process by which scientific
discoveries are made. Our aim is to
let you
meet the leading scientists in
biology, so that you can find out
how
they think about scientific
questions and conduct their
research, and
can get a sense of their
personalities, opinions, and
perspectives. We
also seek to support educators who
want to incorporate materials that
illustrate the process and practice
of science into their curriculum.
This project is made possible by the
good will of many biologists who
are committed to making their work
broadly accessible and to conveying
the excitement of biology to a
worldwide audience.
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www.iBiology.org
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Identification
of
MRSA using 3-colour Crystal digital
PCR
Tuesday, October 24, 2017
8 AM PDT | 11
AM EDT |
4 PM BST | 5 PM CEST |
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Methicillin
resistant
Staphylococcus aureus (MRSA) is a
major pathogen associated
with complicating hospital and
community medicine. Drug resistance
increases the risk of complications
and can challenge routine
procedures such as surgery. As
different species, such as
Staphylococcus epidermidis, can also
carry resistance, this can further
increase the challenge of diagnosis
using culture and/or molecular
methods.
This webcast describes a digital PCR
approach that uses a three colour
detection platform (Naica Crystal
Digital PCR System, Stilla
Technologies) to count the number of
genes that confer resistance and
compare this with the number of genes
from S. aureus and S. epidermidis
within a sample. This method may be
useful to determine which organism
is carrying the resistance gene.
During this webcast you will:
- Understand 3-colour
multiplexing digital
PCR
- Understand The Naica System
Workflow
- Learn a new method to
determine
resistance gene and drug
resistance
- Given a practical study :
identification
of MRSA with the Naica System
- Learn about digital PCR for
infectious
diseases applications
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Nature
-
Podcast Podcast
-
index page
Nature
- Video Streaming
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Science
- Podcast
Science
- Multimedia
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Animations
of Inhibitory RNA Action:
- Nature Reviews
Genetics - Nature Reviews
Genetics
(2001)
"Post-Transcriptional Gene
Silencing by Double-Stranded RNA"
- Nature Reviews
- A high quality movie describing
inhibitory RNA events and
mechanisms
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qPCR
talks around the world:
Reliable
Quantification
by qPCR
a talk by Steve Bustin
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RNA
Quantification in Research and
Diagnostics
a talk by Mikael Kubista
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The Importance of Controls and Novel
Solutions for Successful Real-time
qPCR
a talk by Qiagen
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Learn Why You Might Be One of the 90%
Using
Unsuitable qPCR Reference Genes
a talk by Bio-Rad
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Six Tips for Increasing the
Reproducibility
of qPCR Experiments
a talk by Bio-Rad
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How to Minimize Contamination in qPCR
Experiments
a talk by Bio-Rad
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Quantitative PCR
by Matthias Dobbelstein
Molecular Oncology, GZMB,
Universitätsmedizin Göttingen, Germany
9
videos on YouTube
Quantitative PCR. This series of
short
chalk-talk videos will cover the
principles and calculations associated
with quantitative PCR. The
seminar is also part of a methods
course provided to PhD students of
the Göttingen Graduate School of
Neurosciences and Molecular
Biosciences, GGNB.
- Quantitative PCR -
Introduction A
2:35
- Quantitative PCR -
Introduction B
14:37
- Quantitative PCR -
real-time PCR and
SybrGreen fluorescence 14:05
- Quantitative PCR - the
deltaCt
method 9:27
- Quantitative PCR - deltaCt
in the real
world 10:21
- Quantitative PCR -
RT-PCR 7:36
- Quantitative PCR - internal
controls 8:54
- Quantitative PCR - the
deltadeltaCt
method 6:22
- Quantitative PCR - the
melting
curve 11:25
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Molecular
Biology On-Demand Webinars:
- Direct PCR: Amplify Without
Purification
- PCR Reaction Setup and
Cycling
- Optimize Your PCR
- The Essentials of Nucleic
Acid Sample
Preparation
- How to Avoid Common
Mistakes in the qPCR
Workflow
- Your Key to Success in NGS:
The Library
Preparation Workflow
- DNA Polymerases at Work:
Finding Your
PCR Champion
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Molecular
Biology On-Demand Webinars:
- Restriction Enzyme
Digestion: Cutting
DNA the Right Way
- Optimize Your Cloning: No
More Trouble
With Ligation
- The Essentials of Real-Time
PCR
- Cloning Workflow
Considerations
- Direct PCR
- Thermo Scientific Direct
PCR Kits and
Applications
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Nanostring Webinars
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WEBINAR
-- Characterizing the Performance
of qPCR Instruments – Approaches for
Assessment and Comparison
The
breadth
of instruments available for
quantitative PCR (qPCR) has
continued to grow in the past 5-10
years. With older platforms now
being retired and an abundance of
new technologies available to
replace
them, lab managers, technicians, and
researchers will need to
effectively compare and evaluate the
performance of these platforms.
While new features such as
multiplexing, microfluidics, and
integration
with liquid handling automation have
enabled higher throughput and
lower operating costs, it has made
it increasing complex to readily
compare different types of
instruments and their respective
performance
characteristics.
As
the list
of features and specifications
grows, understanding some of
the key metrics of instrument
performance will become critical for
evaluating platforms that will best
meet the needs of a laboratory’s
application focus and assay
requirements. Unfortunately,
instrument
vendors have not consistently
conformed to any particular
standards for
defining and assessing performance
characteristics of qPCR instruments
and rarely have the methods been
adequately documented in the product
literature.
In
this
webinar, we will define several key
performance metrics of qPCR
instruments such as dynamic range,
Cq uniformity, sensitivity, and
resolution, and further discuss
their importance in practical terms.
Using data from characterization and
verification studies performed on
the IntelliQube instrument from
Douglas Scientific, we will also
review
approaches to evaluating these
metrics, including assays and
software
tools that streamline the analysis
and interpretation of performance
testing results.
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Real-time
PCR
tutorial | qPCR that Works
Scientists worldwide trust real-time
PCR reagents from Takara Bio–even
for the most demanding qPCR
experiments.
When
you
use real-time PCR reagents that are
sensitive and specific, you can
spend less time on PCR troubleshooting
and more time on moving your
research forward. Takara Bio offers
qPCR kits for use with both SYBR
Green detection and TaqMan probes.
Part
1:
Getting You Started
- Find
the right detection method
- Study
your gene expression by RT-qPCR
- Check
the accuracy of your results
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Speaker:
Professor
Mikael
Kubista
TATAA
Biocenter
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Compatible
with
all major real time
PCR instrument systems, these products
allow you to obtain accurate,
consistent results from a wide variety
of sample types, even when other
reagents fail. Want to try Takara Bio
real time PCR reagents for
yourself? Samples are available for
many of our qPCR reagents.
Part 2: Interpreting Your Data
- Determine
Cq
values
- Obtain
absolute quantification of your
starting DNA
- Define
a good reference for relative
quantification
- Follow
MIQE guidelines
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Speaker:
Robert
Sjöback,
Ph.D.
TATAA
Biocenter
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Count
on
Takara Bio to help you choose the best
qPCR products for plant, soil,
blood, paraffin-embedded tissues,
archival or degraded samples or when
conducting gene expression studies,
forensic research analyses, or
other specific applications. Want to
learn more about real-time PCR?
Three videos produced by TATAA
Biocenter in Europe help you
understand
everything from the basics of qPCR to
data analysis techniques. Each
20- to 30-minute video can be watched
at your convenience from the
comfort of your office, lab, or home.
Part 3: Troubleshooting Your
Results
- Identify
potential
pitfalls in qPCR data
- Detect
PCR inhibition
- Control
the quality of RNA and reverse
transcription
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Speaker:
Kristina
Lind,
Ph.D.
TATAA
Biocenter
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Online
Real-Time PCR Training
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Module
1:
Fundamentals
of
real-time PCR |
Module
2:
Assay
design choices |
Module
3:
Normalization |
Module
4:
Multiplexing |
Module
5:
Reagent
choices |
Module
6:
Theory of analysis |
| Access
an
overview of real-time PCR instrument
choices and learn how
real-time PCR differs from endpoint
PCR. How can you use real-time PCR
in your research? What is the
difference between TaqMan® chemistry
and
SYBR® Green chemistry? |
What
should
you consider when planning your gene
expression assays?
What is the impact of
multitranscript assays and assay
specificity? How
do you design custom assays? |
What
is
normalization and why is it
important? What are the types of
normalizers and what are their
functions? How does multigene
normalization work? |
What
is
multiplexing, and when and how do
you use it? |
Discussion
topics
include setting up your real-time
PCR reaction, and preparing
master mixes and reagents for plate
additions. |
Perform
dynamic
range testing with standard curves,
quantify your data
using the ΔΔCt method, and gain a
better understanding of absolute vs.
relative quantification. |
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View
trainings
here |
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Whether you’re new to PCR or need
more in-depth learning guides our
video library will have all the
resources you need. See our
Veriti® Thermal Cycler in action,
catch up with Ph.Diddy and
Ph.Diva and go behind the scenes.
New videos will be added
periodically
so be sure to bookmark this page for
future reference.
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Introduction
to
Real-Time PCR
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Introduction
to
TaqMan® Copy Number Assays
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Introduction
to
TaqMan® Gene Expression Assays
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Introduction
to
TaqMan® Protein Assays
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Fundamentals
of Real-Time PCR |
Introduction
to Digital PCR |
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To
view
these recorded webinars,
please ensure that your pop-up
blocker
is disabled !
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qPCR and MIQE
Seminar Series
"MIQE Oldies but Goodles" from the Sigma
Aldrich Learning Center
As part of our customer education
program, we have
provided two
recorded seminar series covering the topics
of qPCR and MIQE. The
recorded sessions are intended to provide a
high level overview of
these subject matters. We have kept the
lessons concise so that you can
enjoy a self-paced learning program.
| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| Primer
and Probe Design |
Ashley Heath, PhD |
0:06:32 |
| An
Introduction to qPCR Concepts |
Mudassir Mohammed, PhD |
0:09:37 |
| Selecting a
qPCR Basic Detection Chemistry |
Mudassir Mohammed, PhD |
0:12:35 |
| Choosing
a Fluorophore / Quencher Combination |
Anders Bergkvist, PhD |
0:11:30 |
| Chemistries
for More Challenging qPCR Assays |
Mudassir Mohammed, PhD |
0:15:18 |
| MIQE Concepts |
Marina Wiklander, PhD |
0:03:19 |
| Reference
Gene Validation |
Anders Bergkvist, PhD |
0:12:47 |
| Data
Analysis Guidelines |
Anders Bergkvist, PhD |
0:10:42 |
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| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| MIQE: Assay
Design Considerations |
Tania Nolan, PhD |
0:17:37 |
| MIQE: Sample
Derived Inhibitors |
Tania Nolan, PhD |
0:13:04 |
| MIQE: RNA
Quality Considerations |
Tania Nolan, PhD |
0:15:31 |
| MIQE: RNA
Quantity and RT Considerations |
Tania Nolan, PhD |
0:16:38 |
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RNA Integrity
Database (RINdb)- Bioanalyzer
RNA Profiles
Marc Valer, Microfluidics Program
Manager, Genomics,
Agilent
Technologies, Santa Clara.
Synopsis:
Thousands
of users today trust the bioanalyzer 2100
for
qualifying total RNA samples, looking for
integrity, purity, recovery,
and consistency information for sample
preparation methods, assisting
them to determine the optimal conditions
for their experimental design.
Marc Valer describes the use of Agilent's
new RNA Integrity Database
(RINdb) to assist in the development of
experimental protocols,
particularly sample preparations, by
providing a means for researchers
to contextualize RNA profiles.
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Qiagen Webinars
Register for a live Webinar and hear a
live talk about the cutting-edge
technologies of your choice followed
by a Q&A session. The speaker
will answer as many of your questions
as possible during the session.
Any remaining questions will be
answered by personal e-mail after the
Webinar. Alternatively, you can listen
to one of our previously
recorded Webinars.
http://www1.qiagen.com/events/webseminars/default.aspx
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- miRNA purification
and detection — new tools in
expression profiling and biomarker
development
- Recent progress in
RNAi screening
- A successful and
affordable RNAi solution for every
lab
- Novartis scientists
share their experiences in
multiplex, real-time PCR
- Transcriptome-wide
miRNA quantification by RT-PCR
- Accelerate your PCR
and qPCR
- and much
more..........................
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PODCAST:
The Introduction
to Molecular and Cellular Biology lectures
include
text, images, and audio. Each lecture
webpage is synchronized to the
audio
component. In addition, the lectures are
available as a podcast
subscription.
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Fast
PCR and real-time PCR tutorials
(by Bio-Rad)
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The
presentations listed below provide
background and instruction about
specific concepts in PCR.
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Free of
charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series
of webex
seminars dedicated to qPCR. They
will include technical
presentations
from international speakers discussing the
latest techniques and
aspects of qPCR. To register, go
to
QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a
seminar series
devoted to the technical aspects of qPCR and
RT-qPCR.
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Available
Seminars: |
| Author |
Seminar
Title |
| Mudassir
Mohammed, Ph.D. |
An
Introduction to qPCR |
| Valerie
Hawkes, Ph.D. |
Assay Design
and
Optimisation |
| Neven Zoric,
M.Sc. |
Approaches to
Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan,
Ph.D. |
Deriving a
Troubleshooting
Protocol |
| Mikael
Kubista, Prof. |
A Statistical
Approach to
qPCR Gene Expression
Data Analysis |
| Marina
Tshuikina, Ph.D. |
An
Introduction to
Epigenetics Analysis by qPCR |
| Valerie
Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass,
Ph.D. |
Development of
DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito
Disease Vector Populations |
| Michael
Pfaffl, Ph.D. |
Influence of
RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA
Profiling |
| Rebecca Hands |
Technical Tips
on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA
Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak,
M.Sc. |
The SPUD Assay
Has an
Important Role in the
Interpretation of Detecting
Pneumocystis DNA in Clinical
Specimens |
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Transcriptional
Regulation of Eukaryotic Genomes
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Fundamentals
of
Real-Time PCR
(by
Applied
Biosystems,
47 minutes)
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The Eppendorf
real-plex system
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Audio
Slide Show: SmartCycler® System for qPCR
(from
Cepheid)
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RNA
integrity Audio slide shows
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The OpenArray™
Nanotiter Plate Technology and Applications
Understanding
biological complexity arising from patterns
of gene expression requires
accurate and precise measurement of RNA
levels across large numbers of
genes simultaneously. The
preferred method for
quantitative transcriptional analysis is
real time PCR (rt-PCR) in a
96- or 384-well microtiter plate. Scaling
rt-PCR to higher throughputs
is intrinsically limited by cost and
logistic considerations. Alternatively
hybridization
microarrays are capable of measuring
transcription of
many thousands of genes simultaneously yet
are limited by low
sensitivity, accuracy and sample throughput.
We
demonstrate a hybrid approach by combining
the superior accuracy,
precision and dynamic range of rt-PCR with
the high density parallelism
of a microarray in an array of 3,072
real-time, 33 nL polymerase chain
reactions the size of a microscope slide. Real-time
PCR
in this nanotiter plate format results in
substantial savings in
reagents, measurement time and productivity.
We demonstrate system
performance by measuring tissue-specific
expression of kinase genes in
human heart and liver samples and
transcriptional modulation by
small-molecule perturbation of the TNF-alpha
signaling pathway in HUVEC
cells. Compared with the
same assay in a 384-well
microplate, we measured equivalent rt-PCR
performance with a 64-fold
reduction in assay volume, a 24-fold
increase in analytical throughput
and a workflow compatible with standard
microplate protocols in an
easy-to-use fomat.
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Dynamic Arrays to
Measure Expression of Nucleic
Acids and Proteins
Michael
Lucero of
Fluidigm speaking at AMT 2006
Click
here
to launch presentation
A
dynamic array is
a biochip that employs integrated
channels and valves in a matrix
architecture. This matrix architecture
is a breakthrough in efficiency because
performing the same set of
reactions by hand or with a robot would
require orders of magnitude
more sample, reagents, and pipetting
steps.Dynamic
arrays have been introduced that handle
homogeneous or heterogeneous assays, such
as real-time qPCR and ELISAs,
respectively. BioMark™ dynamic arrays for
qPCR accept 48 samples and 48
primer/probe sets. The components are
combined into 2,304 assays (48 x
48).The chip is ideal to
validate expression for 48
genes on samples from many individuals.
Projected throughput of future
chips is ~10,000 reactions. BioMark™
dynamic arrays for immunoassays
accept 48 samples and 18 antibody pairs
and generate 1,728 assays.
Integrated valves prevent mixing between
antibody pairs.Thus,
dynamic arrays prevent signal crosstalk
and
eliminate the need to optimize antibody
pairs for multiplexing in one
vessel, a requirement with other formats.
Instrumentation automates the
loading of chips and analysis of results.
Data produced on BioMark™
dynamic arrays for both applications will
be presented, demonstrating a
sensitivity of detection equivalent to
conventional microwell plates
while generating orders of magnitude
higher throughput.
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A Sequence Oriented
Comparison of Gene Expression
Measurements Across Different
Hybridization-Based Technologies
Winston
Kuo of
Harvard School of Dental Medicine
speaking at AMT 2006
Click
here
to launch presentation
Gene
expression
microarrays have made a profound
impact in biomedical research. The
diversity of platforms and
analytical methods has made comparison of
data from multiple platforms
very challenging. The presentation will
describe a framework for
comparisons across platforms and
laboratories, where probe sequences
were utilized from each vendor to map of
genes across platforms.
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High-throughput
Measurement of Expression Signatures
Using Dynamic Arrays for qPCR and Immunoassays
Michael
Lucero of
Fluidigm speaking at the European
Biomarkers Summit 2006
To purchase a DVD of all the
presentations featured
at the European Biomarkers Summit, please go
to the Select Biosciences
website.
Click
here to launch presentation
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RNAi
screening – advanced tools to accelerate
translational research and
drug discovery
- In
recent
years, RNAi
screening using synthetic siRNA
libraries has become a popular
tool for
elucidating gene functional
pathways and for target
identification.
Find out about the essentials of
RNAi screening and the latest
tools
developed by QIAGEN to enable
its broad application from Dr.
Eric
Lader, QIAGEN’s Associate
Director of RNAi research.
- RNAi screening - advanced tools
to accelerate
translational research and drug
discovery.
- Listen to this exciting Webinar
now.
- RNAi technology and
genome-wide expression
profiling - assessment of
specificity and pathway analysis.
- Listen to this exciting Webinar
now.
- Qiagen
Webinar
web page
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Identification of
New miRNA Markers for Breast Cancer with LNA
Microarrays
Thomas
Litman,
Exiqon, speaking at RNAi Europe 2006
Click here to
launch presentation
Abnormal
expression
of
microRNAs (miRNAs) in cancer implies that
these small ~22-nucleotide
molecules play a role in oncogenesis, and
therefore may comprise a
novel class of diagnostic and prognostic
signatures. Here, we are
studying the global expression profiles of
miRNAs in breast cancer and
adjacent non-malignant breast tissue in
order to identify possible new
biomarkers for breast cancer
Biopsies
from
primary
tumors and from the proximal tissue (1 cm
and 5 cm from the border zone
of tumor) were collected from female
patients (age 55-69) undergoing
surgery for invasive ductal carcinoma.
Total RNA was extracted and
analyzed for global miRNA expression on a
miRCURYTM LNA-based
microarray platform containing capture
probes for over 400 miRNAs.
Our
analysis of
miRNA
expression patterns from tumor and
proximity tissue revealed numerous
differentially expressed miRNAs, including
those reported to be
associated with breast cancer, such as
let-7a/d/f, miR-125a/b, miR-21,
miR-32, and miR-136.
In
addition, we
have
identified several miRNAs that have not
previously been connected with
breast cancer. Some of these novel miRNA
signatures could have
diagnostic and prognostic potential for
breast cancer patients.
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RNAi Based Toold in
Apptosis Research
Yu Shen,
Abbott
Laboratories, speaking at RNAi Europe 2006
Click here to
launch presentation
Several
cases of
off-target gene silencing were identified
in our siRNA library screens
(Lin X et.al. Nucleic Acids Research 33:
4527-35, 2005). However,
despite the complications of off-target
gene silencing, we successfully
identified three cancer targets by
screening an siRNA library against
the “druggable genome” using a cell-death
assay.
In
addition, in an
siRNA-based synthetic lethal screen, we
found that knockdown of
survivin led to the selective killing of
K-Ras mutant cells. We also
explored RNAi-based methodology for target
validation in animal models.
We
developed a
tightly regulated shRNA expression system
(Lin X. et.al. FEBS letter,
577: 376-80, 2004) and used this system to
prepare stable tumor cell
lines that conditionally expressed an
shRNA for HIF-1a. These tumor
cells were implanted in mice to form
tumors that served as a versatile
tool for studying the effects of
inhibiting HIF-1 in vivo (Li L et.al.
Cancer Research, 65: 7249-58, 2005).
Finally,
we
investigated several methods for the
creation of germline knockdown
animals. By modifying the standard
methods, we successfully increased
the transgenic frequency and F1
transmission rate and created
tyrosinase knockdown mice with a
lighter-coat-color phenotype that can
be stably transmitted to the next
generation.
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OpenGenomics Peer
Science
http://www.opengenomics.com/
Welcome
to
the OpenGenomics Peer Science series,
where your colleagues
discuss their latest findings in Genomics
research. With technical
webcasts from your peers, podcasts
providing distilled takes on
research breakthroughs, and the latest in
published papers, Open
Genomics is dedicated to providing fresh
perspectives on pressing
research questions. Check here regularly
for the latest developments in
Genomics.
Published
Papers
A
searchable
database of the latest
publications using Agilent's DNA
microarray platform. Search by
application, date, or keyword.
Webcasts
Each
webcast
contains a short scientific
talk on a specific area of
research, presented by the researcher.
These webcasts are 15-20 minutes
long and are available on demand.
Podcasts
Each
Podcast is
developed as an
informational brief highlighting
innovative approaches to fundamental
research questions, available as
an RSS feed to your IPod®, or available
for listening on your
computer.
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Weekly Podcasts from
the GENcast Network
http://www.genengnews.com/gencasts.aspx
These
weekly
podcasts
feature interviews with leading biotech
researchers, newsmakers, and
thought leaders. Topics revolve around the
critical scientific and
business issues that impact the global
biotechnology industry,
beginning with new discoveries in the lab
and then moving onto R &
D, biomanufacturing, and final product
commercialization. Trends, novel
technologies, opinions, recent
developments, how-to advice, and much
more are discussed in our podcasts in a
lively, to-the-point, and
informative style. New every Thursday, you
can listen right from the
GEN website or you can subscribe using the
button below to have it
download each week automatically to your
iPod or mp3 player!
- THE
INVENTOR
OF PCR - GEN's
Editor-in-Chief John Sterling interviews
the Nobel Prize winning
inventor of PCR, Dr. Kary Mullis.
(2/15/2007) sponsored by: Eppendorf
- RNAi
TECHNOLOGY
- Richard Jorgensen, Ph.D., from
the University of Arizona
(3/22/2007) sponsored by: Sigma-Aldrich
- qPCR
AND
LATE-PCR, AN
ADVANCED TECHNIQUE FOR DNA
AMPLIFICATION - Lawrence
Wangh,
Ph.D., from Brandeis University
(2/22/2007) sponsored
by: Eppendorf
- PROBE-BASED,
REAL-TIME
QUANTITATIVE
PCR ASSAY DESIGN AND APPLICATIONS
- Gregory
L. Shipley, Ph.D., Director,
Quantitative Genomics Core Laboratory,
The
University of Texas Health Science
Center-Houston.
(2/1/2007) sponsored by: Eppendorf
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