eSeminars
& eConference & Webinars
central topics = qPCR
& dPCR & RNAi &
Molecular-Biology
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This
streaming portal is dedicated to scientists from the community of qPCR,
digital PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and
Molecular Diagnostics (MDx). You’ll find here all the records from
around 350 presentations held
at qPCR & NGS and MG Events in the
past years – qPCR 2010 in Vienna to
qPCR dPCR & NGS 2017 in Freising-Weihenstephan, and MicroGenomics
2014
in Paris.
We provide the presentations via movie streaming technology in high
quality – high resolution and perfect sound quality in high speed – on
any internet browser or mobile device, including Android or iOS.
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iBiology
iBiology’s mission is to convey, in the form
of open-access free videos, the excitement of modern biology and the
process by which scientific discoveries are made. Our aim is to let you
meet the leading scientists in biology, so that you can find out how
they think about scientific questions and conduct their research, and
can get a sense of their personalities, opinions, and perspectives. We
also seek to support educators who want to incorporate materials that
illustrate the process and practice of science into their curriculum.
This project is made possible by the good will of many biologists who
are committed to making their work broadly accessible and to conveying
the excitement of biology to a worldwide audience.
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www.iBiology.org
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Identification
of MRSA using 3-colour Crystal digital PCR
Tuesday, October 24, 2017
8 AM PDT | 11 AM EDT |
4 PM BST | 5 PM CEST |
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Methicillin
resistant Staphylococcus aureus (MRSA) is a major pathogen associated
with complicating hospital and community medicine. Drug resistance
increases the risk of complications and can challenge routine
procedures such as surgery. As different species, such as
Staphylococcus epidermidis, can also carry resistance, this can further
increase the challenge of diagnosis using culture and/or molecular
methods.
This webcast describes a digital PCR approach that uses a three colour
detection platform (Naica Crystal Digital PCR System, Stilla
Technologies) to count the number of genes that confer resistance and
compare this with the number of genes from S. aureus and S. epidermidis
within a sample. This method may be useful to determine which organism
is carrying the resistance gene.
During this webcast you will:
- Understand 3-colour multiplexing digital
PCR
- Understand The Naica System Workflow
- Learn a new method to determine
resistance gene and drug resistance
- Given a practical study : identification
of MRSA with the Naica System
- Learn about digital PCR for infectious
diseases applications
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Nature
- Podcast Podcast
- index page
Nature
- Video Streaming
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Science
- Podcast
Science
- Multimedia
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Animations of Inhibitory RNA Action:
- Nature Reviews Genetics - Nature Reviews Genetics
(2001)
"Post-Transcriptional Gene Silencing by Double-Stranded RNA"
- Nature Reviews - A high quality movie describing
inhibitory RNA events and mechanisms
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qPCR
talks around the world:
Reliable
Quantification by qPCR
a talk by Steve Bustin
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RNA Quantification in Research and
Diagnostics
a talk by Mikael Kubista
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The Importance of Controls and Novel
Solutions for Successful Real-time qPCR
a talk by Qiagen
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Learn Why You Might Be One of the 90% Using
Unsuitable qPCR Reference Genes
a talk by Bio-Rad
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Six Tips for Increasing the Reproducibility
of qPCR Experiments
a talk by Bio-Rad
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How to Minimize Contamination in qPCR
Experiments
a talk by Bio-Rad
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Quantitative PCR
by Matthias Dobbelstein
Molecular Oncology, GZMB,
Universitätsmedizin Göttingen, Germany
9
videos on YouTube
Quantitative PCR. This series of short
chalk-talk videos will cover the
principles and calculations associated with quantitative PCR. The
seminar is also part of a methods course provided to PhD students of
the Göttingen Graduate School of Neurosciences and Molecular
Biosciences, GGNB.
- Quantitative PCR - Introduction A
2:35
- Quantitative PCR - Introduction B
14:37
- Quantitative PCR - real-time PCR and
SybrGreen fluorescence 14:05
- Quantitative PCR - the deltaCt
method 9:27
- Quantitative PCR - deltaCt in the real
world 10:21
- Quantitative PCR - RT-PCR 7:36
- Quantitative PCR - internal
controls 8:54
- Quantitative PCR - the deltadeltaCt
method 6:22
- Quantitative PCR - the melting
curve 11:25
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Molecular Biology On-Demand Webinars:
- Direct PCR: Amplify Without Purification
- PCR Reaction Setup and Cycling
- Optimize Your PCR
- The Essentials of Nucleic Acid Sample
Preparation
- How to Avoid Common Mistakes in the qPCR
Workflow
- Your Key to Success in NGS: The Library
Preparation Workflow
- DNA Polymerases at Work: Finding Your
PCR Champion
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Molecular Biology On-Demand Webinars:
- Restriction Enzyme Digestion: Cutting
DNA the Right Way
- Optimize Your Cloning: No More Trouble
With Ligation
- The Essentials of Real-Time PCR
- Cloning Workflow Considerations
- Direct PCR
- Thermo Scientific Direct PCR Kits and
Applications
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Nanostring Webinars
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WEBINAR -- Characterizing the Performance
of qPCR Instruments – Approaches for Assessment and Comparison
The breadth
of instruments available for quantitative PCR (qPCR) has
continued to grow in the past 5-10 years. With older platforms now
being retired and an abundance of new technologies available to replace
them, lab managers, technicians, and researchers will need to
effectively compare and evaluate the performance of these platforms.
While new features such as multiplexing, microfluidics, and integration
with liquid handling automation have enabled higher throughput and
lower operating costs, it has made it increasing complex to readily
compare different types of instruments and their respective performance
characteristics.
As the list
of features and specifications grows, understanding some of
the key metrics of instrument performance will become critical for
evaluating platforms that will best meet the needs of a laboratory’s
application focus and assay requirements. Unfortunately, instrument
vendors have not consistently conformed to any particular standards for
defining and assessing performance characteristics of qPCR instruments
and rarely have the methods been adequately documented in the product
literature.
In this
webinar, we will define several key performance metrics of qPCR
instruments such as dynamic range, Cq uniformity, sensitivity, and
resolution, and further discuss their importance in practical terms.
Using data from characterization and verification studies performed on
the IntelliQube instrument from Douglas Scientific, we will also review
approaches to evaluating these metrics, including assays and software
tools that streamline the analysis and interpretation of performance
testing results.
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Real-time
PCR tutorial | qPCR that Works
Scientists worldwide trust real-time PCR reagents from Takara Bio–even
for the most demanding qPCR experiments.
When
you use real-time PCR reagents that are sensitive and specific, you can
spend less time on PCR troubleshooting and more time on moving your
research forward. Takara Bio offers qPCR kits for use with both SYBR
Green detection and TaqMan probes.
Part
1: Getting You Started
- Find
the right detection method
- Study
your gene expression by RT-qPCR
- Check
the accuracy of your results
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Speaker:
Professor Mikael
Kubista
TATAA Biocenter
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Compatible
with all major real time
PCR instrument systems, these products allow you to obtain accurate,
consistent results from a wide variety of sample types, even when other
reagents fail. Want to try Takara Bio real time PCR reagents for
yourself? Samples are available for many of our qPCR reagents.
Part 2: Interpreting Your Data
- Determine
Cq values
- Obtain
absolute quantification of your starting DNA
- Define
a good reference for relative quantification
- Follow
MIQE guidelines
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Speaker:
Robert
Sjöback, Ph.D.
TATAA Biocenter
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Count on
Takara Bio to help you choose the best qPCR products for plant, soil,
blood, paraffin-embedded tissues, archival or degraded samples or when
conducting gene expression studies, forensic research analyses, or
other specific applications. Want to learn more about real-time PCR?
Three videos produced by TATAA Biocenter in Europe help you understand
everything from the basics of qPCR to data analysis techniques. Each
20- to 30-minute video can be watched at your convenience from the
comfort of your office, lab, or home.
Part 3: Troubleshooting Your Results
- Identify
potential pitfalls in qPCR data
- Detect
PCR inhibition
- Control
the quality of RNA and reverse transcription
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Speaker:
Kristina
Lind, Ph.D.
TATAA Biocenter
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Online Real-Time PCR Training
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Module 1:
Fundamentals of
real-time PCR |
Module 2:
Assay design choices |
Module 3:
Normalization |
Module 4:
Multiplexing |
Module 5:
Reagent choices |
Module 6:
Theory of analysis |
| Access an
overview of real-time PCR instrument choices and learn how
real-time PCR differs from endpoint PCR. How can you use real-time PCR
in your research? What is the difference between TaqMan® chemistry
and
SYBR® Green chemistry? |
What should
you consider when planning your gene expression assays?
What is the impact of multitranscript assays and assay specificity? How
do you design custom assays? |
What is
normalization and why is it important? What are the types of
normalizers and what are their functions? How does multigene
normalization work? |
What is
multiplexing, and when and how do you use it? |
Discussion
topics include setting up your real-time PCR reaction, and preparing
master mixes and reagents for plate additions. |
Perform
dynamic range testing with standard curves, quantify your data
using the ΔΔCt method, and gain a better understanding of absolute vs.
relative quantification. |
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View
trainings here |
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Whether you’re new to PCR or need more in-depth learning guides our
video library will have all the resources you need. See our
Veriti® Thermal Cycler in action, catch up with Ph.Diddy and
Ph.Diva and go behind the scenes. New videos will be added periodically
so be sure to bookmark this page for future reference.
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Introduction
to Real-Time PCR
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Introduction
to TaqMan® Copy Number Assays
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Introduction
to TaqMan® Gene Expression Assays
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Introduction
to TaqMan® Protein Assays
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Fundamentals
of Real-Time PCR |
Introduction
to Digital PCR |
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To
view these recorded webinars,
please ensure that your pop-up blocker
is disabled !
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qPCR and MIQE Seminar Series
"MIQE Oldies but Goodles" from the Sigma Aldrich Learning Center
As part of our customer education program, we have
provided two
recorded seminar series covering the topics of qPCR and MIQE. The
recorded sessions are intended to provide a high level overview of
these subject matters. We have kept the lessons concise so that you can
enjoy a self-paced learning program.
| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| Primer
and Probe Design |
Ashley Heath, PhD |
0:06:32 |
| An
Introduction to qPCR Concepts |
Mudassir Mohammed, PhD |
0:09:37 |
| Selecting a
qPCR Basic Detection Chemistry |
Mudassir Mohammed, PhD |
0:12:35 |
| Choosing
a Fluorophore / Quencher Combination |
Anders Bergkvist, PhD |
0:11:30 |
| Chemistries
for More Challenging qPCR Assays |
Mudassir Mohammed, PhD |
0:15:18 |
| MIQE Concepts |
Marina Wiklander, PhD |
0:03:19 |
| Reference
Gene Validation |
Anders Bergkvist, PhD |
0:12:47 |
| Data
Analysis Guidelines |
Anders Bergkvist, PhD |
0:10:42 |
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| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| MIQE: Assay
Design Considerations |
Tania Nolan, PhD |
0:17:37 |
| MIQE: Sample
Derived Inhibitors |
Tania Nolan, PhD |
0:13:04 |
| MIQE: RNA
Quality Considerations |
Tania Nolan, PhD |
0:15:31 |
| MIQE: RNA
Quantity and RT Considerations |
Tania Nolan, PhD |
0:16:38 |
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RNA Integrity Database (RINdb)- Bioanalyzer
RNA Profiles
Marc Valer, Microfluidics Program Manager, Genomics,
Agilent
Technologies, Santa Clara.
Synopsis:
Thousands of users today trust the bioanalyzer 2100 for
qualifying total RNA samples, looking for integrity, purity, recovery,
and consistency information for sample preparation methods, assisting
them to determine the optimal conditions for their experimental design.
Marc Valer describes the use of Agilent's new RNA Integrity Database
(RINdb) to assist in the development of experimental protocols,
particularly sample preparations, by providing a means for researchers
to contextualize RNA profiles.
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Qiagen Webinars
Register for a live Webinar and hear a live talk about the cutting-edge
technologies of your choice followed by a Q&A session. The speaker
will answer as many of your questions as possible during the session.
Any remaining questions will be answered by personal e-mail after the
Webinar. Alternatively, you can listen to one of our previously
recorded Webinars.
http://www1.qiagen.com/events/webseminars/default.aspx
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- miRNA purification
and detection — new tools in expression profiling and biomarker
development
- Recent progress in
RNAi screening
- A successful and
affordable RNAi solution for every lab
- Novartis scientists
share their experiences in multiplex, real-time PCR
- Transcriptome-wide
miRNA quantification by RT-PCR
- Accelerate your PCR
and qPCR
- and much
more..........................
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PODCAST:
The Introduction
to Molecular and Cellular Biology lectures include
text, images, and audio. Each lecture webpage is synchronized to the
audio
component. In addition, the lectures are available as a podcast
subscription.
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Fast
PCR and real-time PCR tutorials
(by Bio-Rad)
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The
presentations listed below provide background and instruction about
specific concepts in PCR.
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Free of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
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Available Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
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Transcriptional
Regulation of Eukaryotic Genomes
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Fundamentals
of Real-Time PCR
(by
Applied
Biosystems, 47 minutes)
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The Eppendorf
real-plex system
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Audio
Slide Show: SmartCycler® System for qPCR
(from Cepheid)
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RNA
integrity Audio slide shows
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The OpenArray™
Nanotiter Plate Technology and Applications
Understanding
biological complexity arising from patterns of gene expression requires
accurate and precise measurement of RNA levels across large numbers of
genes simultaneously. The preferred method for
quantitative transcriptional analysis is real time PCR (rt-PCR) in a
96- or 384-well microtiter plate. Scaling rt-PCR to higher throughputs
is intrinsically limited by cost and logistic considerations. Alternatively
hybridization microarrays are capable of measuring transcription of
many thousands of genes simultaneously yet are limited by low
sensitivity, accuracy and sample throughput. We
demonstrate a hybrid approach by combining the superior accuracy,
precision and dynamic range of rt-PCR with the high density parallelism
of a microarray in an array of 3,072 real-time, 33 nL polymerase chain
reactions the size of a microscope slide. Real-time PCR
in this nanotiter plate format results in substantial savings in
reagents, measurement time and productivity. We demonstrate system
performance by measuring tissue-specific expression of kinase genes in
human heart and liver samples and transcriptional modulation by
small-molecule perturbation of the TNF-alpha signaling pathway in HUVEC
cells. Compared with the same assay in a 384-well
microplate, we measured equivalent rt-PCR performance with a 64-fold
reduction in assay volume, a 24-fold increase in analytical throughput
and a workflow compatible with standard microplate protocols in an
easy-to-use fomat.
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Dynamic Arrays to
Measure Expression of Nucleic
Acids and Proteins
Michael Lucero of
Fluidigm speaking at AMT 2006
Click
here to launch presentation
A dynamic array is
a biochip that employs integrated
channels and valves in a matrix architecture. This matrix architecture
is a breakthrough in efficiency because performing the same set of
reactions by hand or with a robot would require orders of magnitude
more sample, reagents, and pipetting steps.Dynamic
arrays have been introduced that handle
homogeneous or heterogeneous assays, such as real-time qPCR and ELISAs,
respectively. BioMark™ dynamic arrays for qPCR accept 48 samples and 48
primer/probe sets. The components are combined into 2,304 assays (48 x
48).The chip is ideal to validate expression for 48
genes on samples from many individuals. Projected throughput of future
chips is ~10,000 reactions. BioMark™ dynamic arrays for immunoassays
accept 48 samples and 18 antibody pairs and generate 1,728 assays.
Integrated valves prevent mixing between antibody pairs.Thus,
dynamic arrays prevent signal crosstalk and
eliminate the need to optimize antibody pairs for multiplexing in one
vessel, a requirement with other formats. Instrumentation automates the
loading of chips and analysis of results. Data produced on BioMark™
dynamic arrays for both applications will be presented, demonstrating a
sensitivity of detection equivalent to conventional microwell plates
while generating orders of magnitude higher throughput.
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A Sequence Oriented
Comparison of Gene Expression
Measurements Across Different Hybridization-Based Technologies
Winston Kuo of
Harvard School of Dental Medicine
speaking at AMT 2006
Click
here to launch presentation
Gene expression
microarrays have made a profound
impact in biomedical research. The diversity of platforms and
analytical methods has made comparison of data from multiple platforms
very challenging. The presentation will describe a framework for
comparisons across platforms and laboratories, where probe sequences
were utilized from each vendor to map of genes across platforms.
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High-throughput
Measurement of Expression Signatures
Using Dynamic Arrays for qPCR and Immunoassays
Michael Lucero of
Fluidigm speaking at the European
Biomarkers Summit 2006
To purchase a DVD of all the presentations featured
at the European Biomarkers Summit, please go to the Select Biosciences
website.
Click
here to launch presentation
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RNAi
screening – advanced tools to accelerate translational research and
drug discovery
- In
recent years, RNAi
screening using synthetic siRNA libraries has become a popular tool for
elucidating gene functional pathways and for target identification.
Find out about the essentials of RNAi screening and the latest tools
developed by QIAGEN to enable its broad application from Dr. Eric
Lader, QIAGEN’s Associate Director of RNAi research.
- RNAi screening - advanced tools to accelerate
translational research and drug discovery.
- Listen to this exciting Webinar
now.
- RNAi technology and
genome-wide expression
profiling - assessment of
specificity and pathway analysis.
- Listen to this exciting Webinar
now.
- Qiagen
Webinar web page
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Identification of
New miRNA Markers for Breast Cancer with LNA Microarrays
Thomas Litman,
Exiqon, speaking at RNAi Europe 2006
Click here to launch presentation
Abnormal
expression
of microRNAs (miRNAs) in cancer implies that these small ~22-nucleotide
molecules play a role in oncogenesis, and therefore may comprise a
novel class of diagnostic and prognostic signatures. Here, we are
studying the global expression profiles of miRNAs in breast cancer and
adjacent non-malignant breast tissue in order to identify possible new
biomarkers for breast cancer
Biopsies from
primary
tumors and from the proximal tissue (1 cm and 5 cm from the border zone
of tumor) were collected from female patients (age 55-69) undergoing
surgery for invasive ductal carcinoma. Total RNA was extracted and
analyzed for global miRNA expression on a miRCURYTM LNA-based
microarray platform containing capture probes for over 400 miRNAs.
Our analysis of
miRNA
expression patterns from tumor and proximity tissue revealed numerous
differentially expressed miRNAs, including those reported to be
associated with breast cancer, such as let-7a/d/f, miR-125a/b, miR-21,
miR-32, and miR-136.
In addition, we
have
identified several miRNAs that have not previously been connected with
breast cancer. Some of these novel miRNA signatures could have
diagnostic and prognostic potential for breast cancer patients.
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RNAi Based Toold in
Apptosis Research
Yu Shen, Abbott
Laboratories, speaking at RNAi Europe 2006
Click here to launch presentation
Several cases of
off-target gene silencing were identified in our siRNA library screens
(Lin X et.al. Nucleic Acids Research 33: 4527-35, 2005). However,
despite the complications of off-target gene silencing, we successfully
identified three cancer targets by screening an siRNA library against
the “druggable genome” using a cell-death assay.
In addition, in an
siRNA-based synthetic lethal screen, we found that knockdown of
survivin led to the selective killing of K-Ras mutant cells. We also
explored RNAi-based methodology for target validation in animal models.
We developed a
tightly regulated shRNA expression system (Lin X. et.al. FEBS letter,
577: 376-80, 2004) and used this system to prepare stable tumor cell
lines that conditionally expressed an shRNA for HIF-1a. These tumor
cells were implanted in mice to form tumors that served as a versatile
tool for studying the effects of inhibiting HIF-1 in vivo (Li L et.al.
Cancer Research, 65: 7249-58, 2005).
Finally, we
investigated several methods for the creation of germline knockdown
animals. By modifying the standard methods, we successfully increased
the transgenic frequency and F1 transmission rate and created
tyrosinase knockdown mice with a lighter-coat-color phenotype that can
be stably transmitted to the next generation.
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OpenGenomics Peer
Science
http://www.opengenomics.com/
Welcome to
the OpenGenomics Peer Science series, where your colleagues
discuss their latest findings in Genomics research. With technical
webcasts from your peers, podcasts providing distilled takes on
research breakthroughs, and the latest in published papers, Open
Genomics is dedicated to providing fresh perspectives on pressing
research questions. Check here regularly for the latest developments in
Genomics.
Published Papers
A searchable
database of the latest
publications using Agilent's DNA
microarray platform. Search by application, date, or keyword.
Webcasts
Each webcast
contains a short scientific
talk on a specific area of
research, presented by the researcher. These webcasts are 15-20 minutes
long and are available on demand.
Podcasts
Each Podcast is
developed as an
informational brief highlighting
innovative approaches to fundamental research questions, available as
an RSS feed to your IPod®, or available for listening on your
computer.
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Weekly Podcasts from
the GENcast Network
http://www.genengnews.com/gencasts.aspx
These weekly
podcasts
feature interviews with leading biotech researchers, newsmakers, and
thought leaders. Topics revolve around the critical scientific and
business issues that impact the global biotechnology industry,
beginning with new discoveries in the lab and then moving onto R &
D, biomanufacturing, and final product commercialization. Trends, novel
technologies, opinions, recent developments, how-to advice, and much
more are discussed in our podcasts in a lively, to-the-point, and
informative style. New every Thursday, you can listen right from the
GEN website or you can subscribe using the button below to have it
download each week automatically to your iPod or mp3 player!
- THE INVENTOR
OF PCR - GEN's
Editor-in-Chief John Sterling interviews the Nobel Prize winning
inventor of PCR, Dr. Kary Mullis.
(2/15/2007) sponsored by: Eppendorf
- RNAi
TECHNOLOGY
- Richard Jorgensen, Ph.D., from the University of Arizona
(3/22/2007) sponsored by: Sigma-Aldrich
- qPCR AND
LATE-PCR, AN
ADVANCED TECHNIQUE FOR DNA AMPLIFICATION - Lawrence Wangh,
Ph.D., from Brandeis University (2/22/2007) sponsored
by: Eppendorf
- PROBE-BASED,
REAL-TIME
QUANTITATIVE PCR ASSAY DESIGN AND APPLICATIONS - Gregory
L. Shipley, Ph.D., Director, Quantitative Genomics Core Laboratory, The
University of Texas Health Science Center-Houston.
(2/1/2007) sponsored by: Eppendorf
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for Windows (.wmv)
for Macintosh (.mov)
for
Printing (.pdf)